P09-06

Multi-reconfigurable DNA nanolattice guided by a combination of external stimuli

Yuri KOBAYASHI *, Yuki SUZUKI

Department of Applied Chemistry, Graduate School of Engineering, Mie University
( * E-mail: 423m323@m.mie-u.ac.jp )

The advancements in structural nucleic acids nanotechnology have enabled the construction of various stimuli-responsive nanomachines through molecular self-assembly. These efforts have now extended to the development of multi-reconfigurable nanodevices exhibiting more complex motions, for which operations of distinct movable parts in a combinatorial and reversible manner are required. Here, we report a multi-reconfigurable DNA origami lattice actuator capable of transforming into unique shapes depending on the combination of different types of external cues. The structure consists of nine frames, each composed of a rigid 4-helix bundle connected by flexible single-stranded DNAs. Each frame (except for the central frame) has two bridge strands that form tetraplex structures, such as i-motif or G-quadruplex, in response to pH changes or K+. By regulating tetraplex formations not only with chemical cues but also with their complementary suppressor strands, each frame shape is reversibly reconfigured, enabling the interconversion of different configurations of the lattice actuator. Our simple yet modular design approach will facilitate the development of intelligent biomaterials that exhibit specific transformations in response to combinations of different types of external stimuli.